Our high-throughput, large-scale genome and transcriptome sequencing platform is designed to maximize capacity while maintaining quality, efficiency, scalability and flexibility. We offer several high quality, high throughput targeted sequencing services. Explore your options below, or tells us how we can help.

How can we help?

Clinical

We provide high throughput sequencing for genomic diagnostics in CAP and DAP certified laboratories. The combination of our technical, informatic and clinical expertise ensures that genomic diagnostics will be delivered with high-quality and confidence.

Clinical whole genome sequencing 

The GSC is CAP and DAP certified to provide whole genome sequencing up to the production of BAM files.

Oncopanel  

We have developed the Oncopanel to detect mutations in solid tumors in 45 genes:

AKT1ALKAPCBMPR1ABRAFBRCA1BRCA2CDH1CDK4CDKN2ACICEGFRERBB2 (HER2)ERBB3 (HER3)FUBP1GNAQGNA11HRASIDH1IDH2KITKRASMAP2K1 (MEK1)MET (HGFR)MLH1MSH2MSH6MUTYHNF1NRASPALB2PDGFRAPIK3CAPMS2POLD1POLEPTENRETROS1SDHASDHBSDHCSDHDSMAD4STK11TP53.

Myeloid Panel

We have also developed a Myeloid Panel to detect mutations in 45 genes associated with myeloid malignancies, including acute myeloid leukemia, myeloproliferative neoplasms, and myelodysplastic syndromes:

ASXL1BCORCALRCBLCEBPACSF3RDNMT3AETV6EZH2FLT3GATA2HRASIDH1IDH2JAK2KDM6AKITKMT2A (MLL)KRASMPLNPM1NRASPHF6PIGAPRPF40BPTENPTPN11RAD21RUNX1SETD2SF1SF3A1SF3B1SH2B3SMC1ASMC3SOCS3SRSF2STAG2TET2TP53U2AF1U2AF2WT1ZRSR2.

The current turnaround time is 12-17 days from receipt of sample to mailing of report.

If ordering British Columbia, please refer to the Cancer Genetics Lab website.

If order outside of British Columbia, please contact us for details and requisition forms.

Hereditary Cancer Panel 

Our 17 gene Hereditary Cancer Panel is available to patients across Canada. This panel replaced BRCA1/2 testing in British Columbia in October 2014. The current turnaround time is 8 weeks.

APCBMPR1ABRCA1BRCA2,CDH1MLH1,MSH2MSH6MUTYHPALB2PMS2POLD1POLEPTENSMAD4STK11TP53

A list of ACMG 1, 2, and 3 variants with clinical interpretation will be reported.

Variants in exons 12 and 15 of PMS2 are not reported.

MLPA for copy number changes is offered for BRCA1, BRCA2, MLH1 and MSH2 upon request.

Referrals for this assay must be requested through the BC Cancer Agency Hereditary Cancer Program.

If order outside of British Columbia, please contact us for details and requisition forms.
 

References

The development of our next-generation sequencing assay is described in Bosdet et al. 2013.

Whole Genome

Whole genome sequencing is used for interrogating single-nucleotide variants (SNVs), insertions and deletions (indels), structural variants (SVs), and copy number variants (CNVs) in coding and non-coding regions of the genome.

Options include:

PCR free

  • 30X, 40X, 60X and 80X coverage available

Formalin-fixed paraffin embedded

  • Due to the nature of FFPE samples, this sample type routinely requires more sequences to be generated to reach the same coverage levels as non-FFPE samples.

Chromium

  • Phased whole genome
  • Single Cell RNA sequencing

Long Reads

  • minION  -  Please inquire
  • promethION - Please inquire

RNA

Whole transcriptome RNA sequencing is a next generation sequencing technique that measures the abundance of RNA transcripts in a sample. It is a powerful tool for understanding dynamics in the transcriptome, including gene expression level differences between different physiologic conditions or changes that occur during development or over the course of disease progression.

Options Include

Ribosomal RNA depleted

  • Total RNA-seq with ribosomal reduction selectively removes ribosomal RNA from total RNA samples.

polyA+ RNA

  • Stranded mRNA-Seq is a popular tool for estimating gene expression levels and comparing differential gene expression in model organisms. mRNA-Seq (PolyA+enrichment ssRNA-seq) can also provide valuable information about alternatively spliced variants and can help identify novel isoforms.

Micro RNA

  • miRNA (micro RNA) analysis provides the ability to discover, measure and compare expressions of known microRNAs and other small non-coding RNA.

Single cell

  • Through the 10X Chromium system we can provide single cell sequencing services, including 3’ polyA RNA sequencing and whole genome sequencing aimed at copy number profiling.

Epigenome

Options Include

ChIp

  • Chromatin immunoprecipitation (ChIP) is a powerful experimental approach enabling the identification of proteins associated with specific regions of the genome.

Whole Genome Bisulfite

Methyl Capture

Captures

Whole Exome Sequencing consists of sequencing only a specific subset of the genome, the exons, which represent the entire protein coding part of the genome. Whole Exome sequencing can be used to study genetic variations involved in inherited as well as in sporadic disorders and cancers and are a valid alternative to whole genome sequencing. 

Capture Options: 

  • Exons only
  • IDT 
  • Agilent (V6 + UTR)
  • Custom Capture

Bespoke Projects

Custom Projects and additional services are available upon request. Including submission of constructed libraries. Please connect with us to explore the possibilities.

Constructed Libraries

We also accept constructed libraries, for more information on the requirements please check this document. For anything else please contact us.

Custom Projects

Please contact us to discuss your specific research needs.

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