Morin lab

The Morin Laboratory performs cutting-edge lymphoma research by applying the latest molecular and computational methods in cancer genomics, developing and implementing highly sensitive molecular assays for monitoring circulating tumour DNA, and functionally validating our genomic findings using cell culture work and mouse xenograft models.

More information on the Morin lab is available here.


Comprehensive proteogenomic characterization of mantle cell lymphoma (MCL)

To more completely ascertain the mutations that lead to lymphomagenesis in Mantle cell lymphoma (MCL), Dr. Morin's laboratory is performing an integrative proteogenomic characterization of tumours using a combination of whole genome sequencing, RNA-seq and shotgun proteomics to identify novel coding and non-coding (i.e. regulatory) driver mutations that affect the natural history of MCL including treatment response.

Genetic and molecular mechanisms of treatment resistance in DLBCL

Using comprehensive genomic analyses and by integrating mutation and expression data from over 2000 diffuse large B-cell lymphoma (DLBCL) tumours, Dr. Morin's laboratory seeks novel molecular features that are associated with treatment resistance in DLBCL. The ultimate goal of this work is to develop new predictive and prognostic biomarkers and identify new therapeutic targets for treatment-resistant DLBCLs.

Selected Publications

Integrative genomic analysis of matched primary and metastatic pediatric osteosarcoma.

The Journal of pathology, 2019
Negri, Gian Luca, Grande, Bruno M, Delaidelli, Alberto, El-Naggar, Amal, Cochrane, Dawn, Lau, Ching C, Triche, Timothy J, Moore, Richard A, Jones, Steven Jm, Montpetit, Alexandre, Marra, Marco A, Malkin, David, Morin, Ryan D, Sorensen, Poul H
Despite being the most common childhood bone tumor, the genomic characterization of osteosarcoma remains incomplete. In particular, very few osteosarcoma metastases have been sequenced to date, critical to better understand mechanisms of progression and evolution in this tumor. We performed an integrated whole genome and exome sequencing analysis of paired primary and metastatic pediatric osteosarcoma specimens to identify recurrent genomic alterations. Sequencing of 13 osteosarcoma patients including 13 primary, 10 metastatic, and 3 locally recurring tumors revealed a highly heterogeneous mutational landscape, including cases of hypermutation and microsatellite instability positivity, but with virtually no recurrent alterations except for mutations involving the tumor suppressor genes RB1 and TP53. At the germline level, we detected alterations in multiple cancer related genes in the majority of the cohort, including those potentially disrupting DNA damage response pathways. Metastases retained only a minimal number of short variants from their corresponding primary tumors, while copy number alterations showed higher conservation. One recurrently amplified gene, KDR, was highly expressed in advanced cases and associated with poor prognosis. © 2019 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.

The double-hit signature identifies double-hit diffuse large B-cell lymphoma with genetic events cryptic to FISH.

Blood, 2019
Hilton, Laura K, Tang, Jeffrey, Ben-Neriah, Susana, Alcaide, Miguel, Jiang, Aixiang, Grande, Bruno M, Rushton, Christopher K, Boyle, Merrill, Meissner, Barbara, Scott, David W, Morin, Ryan D

Read our News story here.

High-grade B-cell lymphomas with MYC and BCL2 and/or BCL6 rearrangements (HGBL-DH/THs) include a group of diffuse large B-cell lymphomas (DLBCLs) with inferior outcomes after standard chemoimmunotherapy. We recently described a gene expression signature that identifies 27% of germinal center B-cell DLBCLs (GCB-DLBCLs) as having a double-hit-like expression pattern (DHITsig) and inferior outcomes; however, only half of these cases have both MYC and BCL2 translocations identifiable using standard breakapart fluorescence in situ hybridization (FISH). Here, 20 DHITsig+ GCB-DLBCLs apparently lacking MYC and/or BCL2 rearrangements underwent whole-genome sequencing. This revealed 6 tumors with MYC or BCL2 rearrangements that were cryptic to breakapart FISH. Copy-number analysis identified 3 tumors with MYC and 6 tumors with MIR17HG gains or amplifications, both of which may contribute to dysregulation of MYC and its downstream pathways. Focal deletions of the PVT1 promoter were observed exclusively among DHITsig+ tumors lacking MYC translocations; this may also contribute to MYC overexpression. These results highlight that FISH fails to identify all HGBL-DH/THs, while revealing a range of other genetic mechanisms potentially underlying MYC dysregulation in DHITsig+ DLBCL, suggesting that gene expression profiling is more sensitive for identifying the biology underlying poor outcomes in GCB-DLBCL.

Genome-wide discovery of somatic coding and noncoding mutations in pediatric endemic and sporadic Burkitt lymphoma.

Blood, 2019
Grande, Bruno M, Gerhard, Daniela S, Jiang, Aixiang, Griner, Nicholas B, Abramson, Jeremy S, Alexander, Thomas B, Allen, Hilary, Ayers, Leona W, Bethony, Jeffrey M, Bhatia, Kishor, Bowen, Jay, Casper, Corey, Choi, John Kim, Culibrk, Luka, Davidsen, Tanja M, Dyer, Maureen A, Gastier-Foster, Julie M, Gesuwan, Patee, Greiner, Timothy C, Gross, Thomas G, Hanf, Benjamin, Harris, Nancy Lee, He, Yiwen, Irvin, John D, Jaffe, Elaine S, Jones, Steven J M, Kerchan, Patrick, Knoetze, Nicole, Leal, Fabio E, Lichtenberg, Tara M, Ma, Yussanne, Martin, Jean Paul, Martin, Marie-Reine, Mbulaiteye, Sam M, Mullighan, Charles G, Mungall, Andrew J, Namirembe, Constance, Novik, Karen, Noy, Ariela, Ogwang, Martin D, Omoding, Abraham, Orem, Jackson, Reynolds, Steven J, Rushton, Christopher K, Sandlund, John T, Schmitz, Roland, Taylor, Cynthia, Wilson, Wyndham H, Wright, George W, Zhao, Eric Y, Marra, Marco A, Morin, Ryan D, Staudt, Louis M
Although generally curable with intensive chemotherapy in resource-rich settings, Burkitt lymphoma (BL) remains a deadly disease in older patients and in sub-Saharan Africa. Epstein-Barr virus (EBV) positivity is a feature in more than 90% of cases in malaria-endemic regions, and up to 30% elsewhere. However, the molecular features of BL have not been comprehensively evaluated when taking into account tumor EBV status or geographic origin. Through an integrative analysis of whole-genome and transcriptome data, we show a striking genome-wide increase in aberrant somatic hypermutation in EBV-positive tumors, supporting a link between EBV and activation-induced cytidine deaminase (AICDA) activity. In addition to identifying novel candidate BL genes such as , , and , we demonstrate that EBV-positive tumors had significantly fewer driver mutations, especially among genes with roles in apoptosis. We also found immunoglobulin variable region genes that were disproportionally used to encode clonal B-cell receptors (BCRs) in the tumors. These include IGHV4-34, known to produce autoreactive antibodies, and IGKV3-20, a feature described in other B-cell malignancies but not yet in BL. Our results suggest that tumor EBV status defines a specific BL phenotype irrespective of geographic origin, with particular molecular properties and distinct pathogenic mechanisms. The novel mutation patterns identified here imply rational use of DNA-damaging chemotherapy in some patients with BL and targeted agents such as the CDK4/6 inhibitor palbociclib in others, whereas the importance of BCR signaling in BL strengthens the potential benefit of inhibitors for PI3K, Syk, and Src family kinases among these patients.

A Novel Multiplex Droplet Digital PCR Assay to Identify and Quantify KRAS Mutations in Clinical Specimens.

The Journal of molecular diagnostics : JMD, 2019
Alcaide, Miguel, Cheung, Matthew, Bushell, Kevin, Arthur, Sarah E, Wong, Hui-Li, Karasinska, Joanna, Renouf, Daniel, Schaeffer, David F, McNamara, Suzan, Tertre, Mathilde Couetoux du, Batist, Gerald, Kennecke, Hagen F, Karsan, Aly, Morin, Ryan D
Recurrent activating point mutations in KRAS are critical drivers in pancreatic cancer and have been attributed to resistance to anti-epidermal growth factor receptor therapy in colorectal cancer. Although KRAS genotyping provides limited clinical utility in the diagnosis and management of pancreatic cancer patients at present, inferences about the fractional abundance of KRAS mutations may inform on tumor purity in traditionally challenging clinical specimens and their potential use in precision medicine. KRAS genetic testing has indeed become an essential tool to guide treatment decisions in colorectal cancer, but an unmet need for methods standardization exists. Here, we present a unique droplet digital PCR method that enables the simultaneous detection and quantification of KRAS exon 2, 3, and 4 point mutations and copy number alterations. We have validated 13 mutations (G12S, G12R, G12D, G12A, G12V, G12C, G13D, G60V, Q61H, Q61L, A146V, A146T, and A146P) and focal KRAS amplifications by conducting this assay in a cohort of 100 DNA samples extracted from fresh frozen tumor biopsies, formaldehyde-fixed, paraffin-embedded tissue, and liquid biopsy specimens. Despite its modest lower limit of detection (approximately 1%), this assay will be a rapid cost-effective means to infer the purity of biopsy specimens carrying KRAS mutations and can be used in noninvasive serial monitoring of circulating tumor DNA to evaluate clinical response and/or detect early signs of relapse.

Ultrasensitive Detection of Circulating Tumor DNA in Lymphoma via Targeted Hybridization Capture and Deep Sequencing of Barcoded Libraries.

Methods in molecular biology (Clifton, N.J.), 2019
Alcaide, Miguel, Rushton, Christopher, Morin, Ryan D
Liquid biopsies are rapidly emerging as powerful tools for the early detection of cancer, noninvasive genomic profiling of localized or metastatic tumors, prompt detection of treatment resistance-associated mutations, and monitoring of therapeutic response and minimal residual disease in patients during clinical follow-up. Growing evidence strongly supports the utility of circulating tumor DNA (ctDNA) as a biomarker for the stratification and clinical management of lymphoma patients. However, ctDNA is diluted by variable amounts of cell-free DNA (cfDNA) shed by nonneoplastic cells causing a background signal of wild-type DNA that limits the sensitivity of methods that rely on DNA sequencing. Here, we describe an error suppression method for single-molecule counting that relies on targeted sequencing of cfDNA libraries constructed with semi-degenerate barcode adapters. Custom pools of biotinylated DNA baits for target enrichment can be designed to specifically track somatic mutations in one patient, survey mutation hotspots with diagnostic and prognostic value or be comprised of comprehensive gene panels with broad patient coverage in lymphoma. Such methods are amenable to track ctDNA levels during longitudinal liquid biopsy testing with high specificity and sensitivity and characterize, in real time, the genetic profiles of tumors without the need of standard invasive biopsies. The analysis of ultra-deep sequencing data according to the bioinformatics pipelines also described in this chapter affords to harness lower limits of detection for ctDNA below 0.1%.

Double-Hit Gene Expression Signature Defines a Distinct Subgroup of Germinal Center B-Cell-Like Diffuse Large B-Cell Lymphoma.

Journal of clinical oncology : official journal of the American Society of Clinical Oncology, 2019
Ennishi, Daisuke, Jiang, Aixiang, Boyle, Merrill, Collinge, Brett, Grande, Bruno M, Ben-Neriah, Susana, Rushton, Christopher, Tang, Jeffrey, Thomas, Nicole, Slack, Graham W, Farinha, Pedro, Takata, Katsuyoshi, Miyata-Takata, Tomoko, Craig, Jeffrey, Mottok, Anja, Meissner, Barbara, Saberi, Saeed, Bashashati, Ali, Villa, Diego, Savage, Kerry J, Sehn, Laurie H, Kridel, Robert, Mungall, Andrew J, Marra, Marco A, Shah, Sohrab P, Steidl, Christian, Connors, Joseph M, Gascoyne, Randy D, Morin, Ryan D, Scott, David W
High-grade B-cell lymphoma with MYC and BCL2 and/or BCL6 rearrangements (HGBL-DH/TH) has a poor outcome after standard chemoimmunotherapy. We sought to understand the biologic underpinnings of HGBL-DH/TH with BCL2 rearrangements (HGBL-DH/TH- BCL2) and diffuse large B-cell lymphoma (DLBCL) morphology through examination of gene expression.

Genome-wide discovery of somatic regulatory variants in diffuse large B-cell lymphoma.

Nature communications, 2018
Arthur, Sarah E, Jiang, Aixiang, Grande, Bruno M, Alcaide, Miguel, Cojocaru, Razvan, Rushton, Christopher K, Mottok, Anja, Hilton, Laura K, Lat, Prince Kumar, Zhao, Eric Y, Culibrk, Luka, Ennishi, Daisuke, Jessa, Selin, Chong, Lauren, Thomas, Nicole, Pararajalingam, Prasath, Meissner, Barbara, Boyle, Merrill, Davidson, Jordan, Bushell, Kevin R, Lai, Daniel, Farinha, Pedro, Slack, Graham W, Morin, Gregg B, Shah, Sohrab, Sen, Dipankar, Jones, Steven J M, Mungall, Andrew J, Gascoyne, Randy D, Audas, Timothy E, Unrau, Peter, Marra, Marco A, Connors, Joseph M, Steidl, Christian, Scott, David W, Morin, Ryan D
Diffuse large B-cell lymphoma (DLBCL) is an aggressive cancer originating from mature B-cells. Prognosis is strongly associated with molecular subgroup, although the driver mutations that distinguish the two main subgroups remain poorly defined. Through an integrative analysis of whole genomes, exomes, and transcriptomes, we have uncovered genes and non-coding loci that are commonly mutated in DLBCL. Our analysis has identified novel cis-regulatory sites, and implicates recurrent mutations in the 3' UTR of NFKBIZ as a novel mechanism of oncogene deregulation and NF-κB pathway activation in the activated B-cell (ABC) subgroup. Small amplifications associated with over-expression of FCGR2B (the Fcγ receptor protein IIB), primarily in the germinal centre B-cell (GCB) subgroup, correlate with poor patient outcomes suggestive of a novel oncogene. These results expand the list of subgroup driver mutations that may facilitate implementation of improved diagnostic assays and could offer new avenues for the development of targeted therapeutics.

Frequent mutation of histone-modifying genes in non-Hodgkin lymphoma.

Nature, 2011
Morin, Ryan D, Mendez-Lago, Maria, Mungall, Andrew J, Goya, Rodrigo, Mungall, Karen L, Corbett, Richard D, Johnson, Nathalie A, Severson, Tesa M, Chiu, Readman, Field, Matthew, Jackman, Shaun, Krzywinski, Martin, Scott, David W, Trinh, Diane L, Tamura-Wells, Jessica, Li, Sa, Firme, Marlo R, Rogic, Sanja, Griffith, Malachi, Chan, Susanna, Yakovenko, Oleksandr, Meyer, Irmtraud M, Zhao, Eric Y, Smailus, Duane, Moksa, Michelle, Chittaranjan, Suganthi, Rimsza, Lisa, Brooks-Wilson, Angela, Spinelli, John J, Ben-Neriah, Susana, Meissner, Barbara, Woolcock, Bruce, Boyle, Merrill, McDonald, Helen, Tam, Angela, Zhao, Yongjun, Delaney, Allen, Zeng, Thomas, Tse, Kane, Butterfield, Yaron, Birol, Inanç, Holt, Rob, Schein, Jacqueline, Horsman, Douglas E, Moore, Richard, Jones, Steven J M, Connors, Joseph M, Hirst, Martin, Gascoyne, Randy D, Marra, Marco A
Follicular lymphoma (FL) and diffuse large B-cell lymphoma (DLBCL) are the two most common non-Hodgkin lymphomas (NHLs). Here we sequenced tumour and matched normal DNA from 13 DLBCL cases and one FL case to identify genes with mutations in B-cell NHL. We analysed RNA-seq data from these and another 113 NHLs to identify genes with candidate mutations, and then re-sequenced tumour and matched normal DNA from these cases to confirm 109 genes with multiple somatic mutations. Genes with roles in histone modification were frequent targets of somatic mutation. For example, 32% of DLBCL and 89% of FL cases had somatic mutations in MLL2, which encodes a histone methyltransferase, and 11.4% and 13.4% of DLBCL and FL cases, respectively, had mutations in MEF2B, a calcium-regulated gene that cooperates with CREBBP and EP300 in acetylating histones. Our analysis suggests a previously unappreciated disruption of chromatin biology in lymphomagenesis.

Somatic mutations altering EZH2 (Tyr641) in follicular and diffuse large B-cell lymphomas of germinal-center origin.

Nature genetics, 2010
Morin, Ryan D, Johnson, Nathalie A, Severson, Tesa M, Mungall, Andrew J, An, Jianghong, Goya, Rodrigo, Paul, Jessica E, Boyle, Merrill, Woolcock, Bruce W, Kuchenbauer, Florian, Yap, Damian, Humphries, R Keith, Griffith, Obi L, Shah, Sohrab, Zhu, Henry, Kimbara, Michelle, Shashkin, Pavel, Charlot, Jean F, Tcherpakov, Marianna, Corbett, Richard, Tam, Angela, Varhol, Richard, Smailus, Duane, Moksa, Michelle, Zhao, Yongjun, Delaney, Allen, Qian, Hong, Birol, Inanc, Schein, Jacqueline, Moore, Richard, Holt, Robert, Horsman, Doug E, Connors, Joseph M, Jones, Steven, Aparicio, Samuel, Hirst, Martin, Gascoyne, Randy D, Marra, Marco A
Follicular lymphoma (FL) and the GCB subtype of diffuse large B-cell lymphoma (DLBCL) derive from germinal center B cells. Targeted resequencing studies have revealed mutations in various genes encoding proteins in the NF-kappaB pathway that contribute to the activated B-cell (ABC) DLBCL subtype, but thus far few GCB-specific mutations have been identified. Here we report recurrent somatic mutations affecting the polycomb-group oncogene EZH2, which encodes a histone methyltransferase responsible for trimethylating Lys27 of histone H3 (H3K27). After the recent discovery of mutations in KDM6A (UTX), which encodes the histone H3K27me3 demethylase UTX, in several cancer types, EZH2 is the second histone methyltransferase gene found to be mutated in cancer. These mutations, which result in the replacement of a single tyrosine in the SET domain of the EZH2 protein (Tyr641), occur in 21.7% of GCB DLBCLs and 7.2% of FLs and are absent from ABC DLBCLs. Our data are consistent with the notion that EZH2 proteins with mutant Tyr641 have reduced enzymatic activity in vitro.

Open Positions

Postdoctoral Fellow – Integrative Genomic Analysis of Lymphoid Cancers

About us
Canada's Michael Smith Genome Sciences Centre (GSC) at BC Cancer is an international leader in genomics, proteomics and bioinformatics for precision medicine. By developing and deploying genome sequencing, computational and analytical technology, we are creating novel strategies to prevent and diagnose cancers and other diseases, uncovering new therapeutic targets and helping the world realize the social and economic benefits of genome science

Learn more about how the GSC is Bringing Genomics to Life.

Why work at the GSC
As the first genome centre to be established within a cancer clinic, our story began by thinking outside of the box. From being the first in the world to sequence the SARS coronavirus during the 2003 global outbreak to publishing the first study demonstrating the use of whole genome sequencing to influence personalized cancer treatment planning in 2010, our passion for pushing scientific frontiers continues to this day.

Our technology platforms enable the transformative science being done at the GSC. But it is our world-class team of scientists and innovators that make it happen. By joining the GSC you will become part of a diverse and dedicated group of biologists, bioinformaticians, computer scientists, computational biologists, biochemists, engineers and clinicians. We look for people who share our core values—science, timeliness and respect—to join us in our mission to provide genomics, bioinformatics and proteomics technology and expertise for the benefit of human health and society.

We believe that diversity and inclusivity is essential for the advancement of human knowledge and science. We welcome all applicants and provide all employees with equal opportunity for advancement, regardless of race, colour, ancestry, place of origin, political belief, religion, marital status, family status, physical or mental disability, sex, sexual orientation, gender identity or expression, age, conviction of a criminal or summary conviction offence unrelated to their employment

All qualified candidates are encouraged to apply; however, Canadian citizens and permanent residents will be given priority.

Postdoctoral Fellow
Lab/group: Ryan Morin Lab  

Role Summary
The Morin and Scott laboratories are seeking a Postdoctoral Fellow to take a leadership role in an ongoing effort to resolve the molecular aetiology of aggressive lymphoid cancers using genomic techniques. This individual will work closely with a team of bioinformaticians, biostatisticians and clinician-scientists in a highly productive and stimulating research environment at a world-class research facility in Vancouver, Canada.

The successful applicant will apply cutting-edge bioinformatic techniques to analyze hundreds of terabytes of high-throughput sequencing data produced from clinical cancer samples, namely RNA-seq (bulk/single cell), whole exome, whole genome, and circulating tumour DNA sequencing data. This position demands a strong background in bioinformatics, computational biology or data science. Detailed knowledge of cancer biology (particularly non-Hodgkin lymphomas) would be an asset.

Familiarity with algorithms for inferring clonal structure of tumours and network/pathway deregulation from sequence data is also desirable. Experience with machine learning, statistical modelling, survival analysis, and data visualization are all desirable. Characteristics of the ideal candidate include strong verbal and written communication skills, attention to detail, creativity, and the ability to remain organized in a fast-paced and dynamic environment. The successful candidate will thrive in our highly collaborative setting while also being able to drive projects independently.

Diversity is an integral part of Canadian history, culture, and identity. In the Morin laboratory, we strive to maintain an equitable and inclusive culture where all forms of diversity are seen as added value in the unifying goal of reducing the impact of cancer.

The successful candidate will report to Drs. ​Ryan Morin​ and David Scott and will be affiliated with Canada’s Michael Smith Genome Sciences Centre at BC Cancer.

Key Responsibilities :
Duties will include some combination of the following:

  • Deploy pipelines to detect somatic alterations such as CNAs, SNVs, indels and structural alterations/fusion transcripts
  • Perform clustering analyses on mutational and/or gene expression features to identify novel subgroupings
  • Develop machine learning models for the accurate identification of somatic mutations in heterogeneous and/or noisy tumour samples (e.g. FFPE, unpaired, ctDNA)
  • Identify cis-regulatory mutations using copy number, SV, and non-coding mutations
  • Quantify mutational signatures de novo or using reference signatures for simple somatic mutations and SVs
  • Model the effect of coding and non-coding mutations, respectively, on protein and RNA structure and function
  • Resolve clonal substructure and track tumour evolution using tissue biopsies and ctDNA
  • Identify gene expression or splicing features associated with clinical or genetic variables
  • Create bespoke visualizations of high-dimensional genomic datasets
  • Design and execute experiments and disseminate results through conference presentations and publications
  • Contribute to the writing of grant applications and training of junior lab members

Suitable candidates should possess a PhD degree in bioinformatics, computer science, or molecular biology and must be competent in at least one programming language such as Python or R.

To apply
Applicants are requested to send a cover letter and CV to with LYMPHOMA-PDF in the subject line




Christiana (Tiana) Carstairs

Projects Manager


Bruno Grande

Graduate Student
Back to top