RNA-Bloom v1.3.1 args: [-t, 12, -ntcard, -outdir, /my/output/dir, -long, /my/input/dir/nanosim_mouse_rna.fa.gz] name: rnabloom outdir: /my/output/dir WARNING: Output directory does not exist! Created output directory at `/my/output/dir` K-mer counting with ntCard... Running command: `ntcard -t 12 -k 17 -c 65535 -p /my/output/dir/rnabloom @/my/output/dir/rnabloom.ntcard.readslist.txt`... Parsing histogram file `/my/output/dir/rnabloom_k17.hist`... Unique k-mers (k=17): 337,491,027 Min k-mer coverage threshold: 3 K-mer counting completed in 17.398s Bloom filters Memory (GB) ==================================== de Bruijn graph: 0.7458036 k-mer counting: 0.7842373 ==================================== Total: 1.530041 > Stage 1: Construct graph from reads (k=17) [1] Parsing `/my/input/dir/nanosim_mouse_rna.fa.gz`... [1] Parsed 442,245 sequences. Parsed 442,245 reads in total. DBG Bloom filter FPR: 0.99947804 % Counting Bloom filter FPR: 1.045231 % > Stage 1 completed in 3m 13s > Stage 2: Correct long reads for "rnabloom" Parsing `/my/input/dir/nanosim_mouse_rna.fa.gz`... Corrected Read Lengths Sampling Distribution (n=1000) min q1 med q3 max 207 764 1127 1499 15641 Parsed 442,245 sequences. Kept: 409,197(92.52722%) Discarded: 33,048(7.4727807%) Artifacts: 83(0.018767878%) > Stage 2 completed in 9m 11s > Stage 3: Assemble long reads for "rnabloom" Overlapped sequences: 373,549 - discarded: 12,119 - unique: 19,223 - dovetail: 4,916 G: |V|=9,832 |E|=21,088 G: |V|=9,832 |E|=5,936 before: 408,972 after: 16,255 > Stage 3 completed in 25m 53s Total runtime: 38m 35s