-------------------------------------------------------------------------------- SUMMARY -------------------------------------------------------------------------------- - Mon May 29 00:31:50 2017 - [A67711]- commit hash = b2fcf38 - assembly checksum = 1,312,056,501,871,170,731 -------------------------------------------------------------------------------- INPUT - 503.80 M = READS = number of reads; ideal 800-1200 for human - 139.00 b = MEAN READ LEN = mean read length after trimming; ideal 140 - 62.79 x = EFFECTIVE COV = effective read coverage; ideal ~42 for nominal 56x cov - 61.49 % = READ TWO Q30 = fraction of Q30 bases in read 2; ideal 75-85 - 0.41 kb = MEDIAN INSERT = median insert size; ideal 0.35-0.40 - 80.39 % = PROPER PAIRS = fraction of proper read pairs; ideal >=75 - 30.19 kb = MOLECULE LEN = weighted mean molecule size; ideal 50-100 - 0.87 kb = HETDIST = mean distance between heterozygous SNPs - 8.11 % = UNBAR = fraction of reads that are not barcoded - 426.00 = BARCODE N50 = N50 reads per barcode - 35.54 % = DUPS = fraction of reads that are duplicates - 21.90 % = PHASED = nonduplicate and phased reads; ideal 45-50 -------------------------------------------------------------------------------- OUTPUT - 1.38 K = LONG SCAFFOLDS = number of scaffolds >= 10 kb - 2.04 kb = EDGE N50 = N50 edge size - 36.12 kb = CONTIG N50 = N50 contig size - 0.10 Mb = PHASEBLOCK N50 = N50 phase block size - 0.94 Mb = SCAFFOLD N50 = N50 scaffold size - 0.69 Mb = SCAFFOLD N60 = N60 scaffold size - 0.38 Gb = ASSEMBLY SIZE = assembly size (only scaffolds >= 10 kb) --------------------------------------------------------------------------------