-------------------------------------------------------------------------------- SUMMARY -------------------------------------------------------------------------------- - Fri May 12 18:45:24 2017 - [A67643]- commit hash = b2fcf38 - assembly checksum = 1,104,592,063,549,560,064 -------------------------------------------------------------------------------- INPUT - 516.05 M = READS = number of reads; ideal 800-1200 for human - 139.00 b = MEAN READ LEN = mean read length after trimming; ideal 140 - 86.44 x = EFFECTIVE COV = effective read coverage; ideal ~42 for nominal 56x cov - 62.95 % = READ TWO Q30 = fraction of Q30 bases in read 2; ideal 75-85 - 0.42 kb = MEDIAN INSERT = median insert size; ideal 0.35-0.40 - 78.52 % = PROPER PAIRS = fraction of proper read pairs; ideal >=75 - 45.05 kb = MOLECULE LEN = weighted mean molecule size; ideal 50-100 - 0.97 kb = HETDIST = mean distance between heterozygous SNPs - 6.73 % = UNBAR = fraction of reads that are not barcoded - 380.00 = BARCODE N50 = N50 reads per barcode - 24.65 % = DUPS = fraction of reads that are duplicates - 24.00 % = PHASED = nonduplicate and phased reads; ideal 45-50 -------------------------------------------------------------------------------- OUTPUT - 0.84 K = LONG SCAFFOLDS = number of scaffolds >= 10 kb - 2.02 kb = EDGE N50 = N50 edge size - 38.73 kb = CONTIG N50 = N50 contig size - 1.11 Mb = PHASEBLOCK N50 = N50 phase block size - 1.54 Mb = SCAFFOLD N50 = N50 scaffold size - 1.28 Mb = SCAFFOLD N60 = N60 scaffold size - 0.39 Gb = ASSEMBLY SIZE = assembly size (only scaffolds >= 10 kb) --------------------------------------------------------------------------------