-------------------------------------------------------------------------------- SUMMARY -------------------------------------------------------------------------------- - Thu Jun 08 06:48:29 2017 - [A67645_A67710]- commit hash = b2fcf38 - assembly checksum = 1,245,971,915,084,695,777 -------------------------------------------------------------------------------- INPUT - 541.72 M = READS = number of reads; ideal 800-1200 for human - 139.00 b = MEAN READ LEN = mean read length after trimming; ideal 140 - 75.66 x = EFFECTIVE COV = effective read coverage; ideal ~42 for nominal 56x cov - 61.17 % = READ TWO Q30 = fraction of Q30 bases in read 2; ideal 75-85 - 0.41 kb = MEDIAN INSERT = median insert size; ideal 0.35-0.40 - 79.91 % = PROPER PAIRS = fraction of proper read pairs; ideal >=75 - 33.64 kb = MOLECULE LEN = weighted mean molecule size; ideal 50-100 - 0.89 kb = HETDIST = mean distance between heterozygous SNPs - 7.11 % = UNBAR = fraction of reads that are not barcoded - 290.00 = BARCODE N50 = N50 reads per barcode - 27.60 % = DUPS = fraction of reads that are duplicates - 20.76 % = PHASED = nonduplicate and phased reads; ideal 45-50 -------------------------------------------------------------------------------- OUTPUT - 1.91 K = LONG SCAFFOLDS = number of scaffolds >= 10 kb - 1.59 kb = EDGE N50 = N50 edge size - 30.44 kb = CONTIG N50 = N50 contig size - 0.06 Mb = PHASEBLOCK N50 = N50 phase block size - 0.56 Mb = SCAFFOLD N50 = N50 scaffold size - 0.41 Mb = SCAFFOLD N60 = N60 scaffold size - 0.35 Gb = ASSEMBLY SIZE = assembly size (only scaffolds >= 10 kb) --------------------------------------------------------------------------------