Running: ../LINKS [v1.5] -f RK12links29.scaffolds.fa -s K12_R.fof -d 16000 -k 15 -e 0.1 -l 5 -a 0.3 -t 2 -o 0 -b RK12links30 -r RK12links1.bloom -g 0 Checking K12_rawONT_longread.fa...ok Checking sequence target file RK12links29.scaffolds.fa...ok =>Reading contigs file, building Bloom filter: Mon Apr 27 16:47:03 PDT 2015 Checking Bloom filter file RK12links1.bloom...ok A Bloom filter was supplied (RK12links1.bloom) and will be used instead of building a new one from -f RK12links29.scaffolds.fa =>Reading long reads, building hash table: Mon Apr 27 16:47:03 PDT 2015 Reads processed k=15, dist=16000, offset=0 nt, sliding step=2 nt: Reads processed from file 1/1, K12_rawONT_longread.fa: 70531 Extracted 1400 total 15-mer pairs =>Reading sequence contigs (to scaffold), tracking k-mer positions: Mon Apr 27 16:47:10 PDT 2015 Contigs processed k=15: 28 =>Scaffolding initiated: Mon Apr 27 16:47:13 PDT 2015 ===========PAIRED K-MER STATS=========== Total number of pairs extracted from -s K12_R.fof: 1400 At least one sequence/pair missing from contigs: 0 Assembled pairs: 1175 (2350 sequences) Satisfied in distance/logic within contigs (i.e. -> <-, distance on target: 727 Unsatisfied in distance within contigs (i.e. distance out-of-bounds): 59 Unsatisfied pairing logic within contigs (i.e. illogical pairing ->->, <-<- or <-->): 58 --- Satisfied in distance/logic within a given contig pair (pre-scaffold): 1 Unsatisfied in distance within a given contig pair (i.e. calculated distances out-of-bounds): 330 --- Total satisfied: 728 unsatisfied: 447 Breakdown by insert sizes: --------Reads with 16000 bp inserts-------- MIN:14400 MAX:17600 as defined by 16000 * 0.1 At least one sequence/pair missing: Assembled pairs: 1175 Satisfied in distance/logic within contigs (i.e. -> <-, distance on target: 727 Unsatisfied in distance within contigs (i.e. distance out-of-bounds): 59 Unsatisfied pairing logic within contigs (i.e. illogical pairing ->->, <-<- or <-->): 58 --- Satisfied in distance/logic within a given contig pair (pre-scaffold): 1 Unsatisfied in distance within a given contig pair (i.e. calculated distances out-of-bounds): 330 ============================================ =>Scaffolding ended: Mon Apr 27 16:47:15 PDT 2015 =>Making FASTA file: Mon Apr 27 16:47:15 PDT 2015 Scaffolding executed normally Mon Apr 27 16:47:16 PDT 2015