FastQCFastQC Report
Thu 20 Aug 2015
read1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filenameread1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124291599
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCG71800.024.78873365
CGATCGG96450.018.88243366
ACCCGAT126700.014.29405463
ATCGGCA162950.011.19782568
CGTACGC87450.010.37283310
GCCGAAC196550.09.69141145
GATCGGC203350.09.54983567
CCGTACG95850.09.4997749
CCGAACT202350.09.3799746
CACCCGA201950.09.34395162
ACTTAGT205050.09.27324850
CCCGATC228650.08.47805264
ACACCCG240350.08.13848761
TACGCCA120850.07.934135412
TTCGGCG123950.07.628570649
TGCGGAC261200.07.56978256
GAACTTA258250.07.563535748
AACTTAG261000.07.35145749
TAGTGCG261350.07.23584153
CGAGCGA85900.07.190544656
Produced by FastQC (version 0.11.3)