FastQCFastQC Report
Thu 12 Dec 2019
lane8_TAGCTT_S30_L008_I1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane8_TAGCTT_S30_L008_I1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences229259278
Sequences flagged as poor quality0
Sequence length6
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCTT22186263996.77367953675576No Hit
TCGCTT19820040.8645250989580453No Hit
TAGCTG14609130.6372317895897762No Hit
NAGCTT6930870.30231579111925844No Hit
TAGCTA6236720.2720378452906058No Hit
TAGTTT6002530.2618227734277345No Hit
TAGCCT4406740.1922164301677684No Hit
TAGCGT3756140.16383808030661248No Hit
AAGCTT2357330.10282375573040059No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.8)