MeDIP - Methylated DNA immunoprecipitation (MeDIP) is a versatile immunocapturing approach for unbiased detection of methylated DNA. First, genomic DNA is randomly fragmented using sonication and then immunoprecipitated with a monoclonal antibody that specifically recognizes 5-methylcytidine. Next the precipitated DNA fragments are sequenced (using either single-end or pair-end sequencing), and then aligned to the reference genome. The coverage of these reads indicates genomic regions with methylated CpGs. The efficiency of MeDIP is higher in genomic regions with greater CpG density.
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