Job Reference No. RA1/2_R00003/4_Holt Lab_2022_03_07

Canada’s Michael Smith Genome Sciences Centre (GSC) is seeking a Research Technician to assist in conducting a 12-month, industry-facing, translational research project to validate the commercial potential of a high-throughput platform for the discovery and characterization of therapeutic T cell receptors (TCRs).

Tope-seq, developed by Dr. Robert Holt and his research team, is a patented cell-based assay that allows TCR reactivity to be screened in vitro against T cell antigen libraries expressed in synthetic antigen presenting cells. This allows TCR reactivity to be characterized in both a high-throughput and functionally-relevant manner; a combination of capabilities unmatched by current and emerging technologies. The Tope-seq platform aims to de-risk the development of TCR-based immunotherapies by increasing the likelihood that they achieve success in clinical trials, and ultimately, save lives.

The goal of this objective-based project is to use a series of defined experiments to generate data that will be critical in the further development of the Tope-seq platform, and its translation to a commercial environment.

Project areas: Immunogenomics, T cell biology, engineered cell therapy, and synthetic biology.

Duties and Responsibilities

As a Research Technician on the Tope-seq project, you will report to Dr. Robert Holt and the Tope-seq Team. Your typical duties will be to perform SOP-driven activities that include the following:

  • Maintaining engineered cell lines.
  • Assisting in the generation of molecular tools and viral vectors.
  • Assisting in high-throughput T cell receptor profiling screens.
  • Assisting in the characterization and validation of novel discoveries stemming from high-throughput screening studies.
  • Reporting research methods and results in team meetings.


The ideal candidate should possess a MSc degree, or BSc degree with 3+ years of experience, and experience in immunology, genomics, cell biology or a related discipline.

Experience in recombinant DNA technologies and viral gene delivery vectors, mammalian cell culture (including primary T cell culture), and the preparation of Illumina sequencing libraries is desired. Familiarity with flow cytometry/FACS and cell-based assays (such as ELISPOT) will be an asset.

Experience working on focused objective-based research projects and a track record of success in team-based environments is highly desirable.

Position and compensation will be commensurate with experience.


Please submit a detailed cover letter and resume to, using Job Reference No. RA1/2_R00003/4_Holt Lab_2022_03_07 in the subject line of your email.

This posting will remain online until filled.

As per the current Public Health Order, full vaccination against COVID-19 is a condition of employment with PHSA as of October 26, 2021

We believe that equity, diversity and inclusivity are essential for the advancement of human knowledge and science.

We welcome all applicants and provide all employees with equal opportunity for advancement, regardless of race, colour, ancestry, place of origin, political belief, religion, marital status, family status, physical or mental disability, sex, sexual orientation, gender identity or expression, age, conviction of a criminal or summary conviction offence unrelated to their employment.

All qualified candidates are encouraged to apply; however, Canadian citizens and permanent residents will be given priority.

About the GSC

Canada’s Michael Smith Genome Sciences Centre (GSC)

Today’s Research. Tomorrow’s Medicine.

The GSC is a department of the BC Cancer Research Institute and a high-throughput genome sequencing facility. We are leaders in genomics, proteomics and bioinformatics in pursuit of novel treatment strategies for cancers and other diseases.

Among the world’s first genome centres to be established within a cancer clinic, for more than two decades our scientists and innovators have been designing and deploying cutting-edge technologies to benefit health and advance clinical research.

Among the GSC’s most significant accomplishments are the first publication to demonstrate the use of whole-genome sequencing to inform cancer treatment planning, the first published sequence of the SARS coronavirus genome and major contributions to the first physical map of the human genome as part of the Human Genome Project.

By joining the GSC you will become part of an exceptional and diverse team of scientists, clinicians, experts and professionals operating at the leading edge of clinical research. We look for people who share our core values—science, timeliness, respect—to join us on our mission to use genome science for the betterment of health and society.

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