Towards Single Cell Genomics
Integration of multilayer soft lithography with next-generation sequencing in order to achieve transcriptome analysis of single cells.
|Project Leaders||Marco Marra , Carl Hansen|
|Project Co-Investigators||Steven Jones , Martin Hirst , Sam Aparicio , Robert Holt|
Coloured reagents being mixed within the microfluidic chip.
- These are:
- Construction and sequencing microRNA libraries prepared from RNA purified from single cells
- Construction and sequencing of SAGE libraries from RNA purified from single cells
- Construction and sequencing of whole transcriptome sequencing libraries from RNA purified from single cells
- Development of multiplexing strategies for high-throughput single cell profiling
Expected deliverables of this project are microfluidic devices designed to perform single cell manipulations in formats capable of interfacing with next generation sequencing devices. We will also produce protocols to allow replication of our procedures in other laboratories. We anticipate that translation of our technologies to the scientific, biotechnology and pharmaceutical industries will have profound consequences for several fields including biomarker discovery, drug development, environmental genomics, metagenomics and personalized medicine.For all project related inquires please contact us.
Robyn Roscoe, Projects Leader
Genome Sciences Centre, BC Cancer Agency
Phone: (604)707-5900 x 5963