Expression Profiles of Cells and Tissues in C. elegans
Helping researchers discover the function of 2,000 genes identified in the nematode that are similar to genes in humans.
|Project Leaders||David Baillie|
|Project Co-Investigators||Don Moerman|
Building upon the first Genome BC C. elegans research project, this study is helping researchers discover the function of 2,000 genes identified in the nematode that are similar to genes in humans. This project plans to describe the gene expression and protein network profiles in C. elegans, the transparent worm used as a model organism to study gene function. By discovering the function of genes in the worm and where they impact cellular processes, this project will develop a better understanding of how the equivalent genes function in humans. Researchers will use a number of techniques, including serial analysis of gene expression, GFP constructs, and yeast 2-hybrid screens to determine the RNA and protein profiles in specific tissues of C. elegans. The GFP constructs show a gene’s expression pattern by making the target cell or organ fluoresce green. By the end of the project in March 2006 more than 2000 expressing GFP constructs will have been made, providing the largest GFP construct collection for C. elegans study worldwide.