Illumina Sequencing Platform
Illumina HiSeq 2000
Currently their are 12 Illumina HiSeq 2000 and 3 Illumina Genome Analyzer IIx performing sequencing at the GSC. Sequencing capabilities include both single-end sequencing (50 and 75bp read lengths) and pair-end sequencing (50, 75 and 100bp read lengths). Library construction for Illumina sequencing is available for whole genome, transcriptome (RNA-seq), exome, microRNA, and chromatin immunoprecipitation (ChIP), as well as for “bar coding” of samples for multiplex sequencing. The GSC has to date constructed over 2,000 libraries and generated over 7.5 x 1013 base pairs of data on the Illumina Genome Analyzer, contributing to many projects and publications.