Summary

To provide a physical map resource to organize sequencing of the rat genome, the Genome Sciences Centre, Vancouver, BC is constructing a database of fingerprinted BAC clones. This effort is funded by the National Institutes of Health (NIH), through the National Human Genome Research Institute (NHGRI) and the National Heart, Lung, and Blood Institute (NHGRI). We aim to generate a total of 200,000 whole-clone HindIII fingerprints from the BAC library CHORI-230 constructed by P. De Jong from the Brown Norway rat (BN/SsNHsd/MCW) DNA. We are using an improved agarose gel methodology which has been used to construct a high-resolution BAC-based fingerprint map of the mouse genome here at the Genome Sequence Centre. BAC end sequencing will be carried out by The Institute for Genome Research. Genomic BAC-based rat sequences will be generated by the joint efforts of the Baylor College of Medicine Human Genome Sequencing Center, Celera Genomics, and Genome Therapeutics. A central repository for the rat genomic resources is maintained at the Rat Genome Database.

Clone Nomenclature