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Mouse
Summary
To provide a physical map resource to organize sequencing of the mouse genome, the Genome Sciences Centre, Vancouver, BC is constructing a database of fingerprinted BAC clones. This effort is funded by the The National Institutes of Health (NIH), through the National Human Genome Research Institute (NHGRI). We aim to generate a total of 300,000 whole-clone HindIII fingerprints from two BAC libraries constructed by P. De Jong from C57BL/6J mouse DNA. One library, RPCI-23 (Osoegawa et al 2000), has been constructed from females, and the other, RPCI-24, is from males of the same strain. We are using the agarose gel methodology developed at Washington University Genome Sequencing Center (Marra et al., 1997), which was recently used to produce BAC-based physical maps of the Arabidopsis thaliana genome (Marra et al., 1999 ; Mozo et al., 1999). BAC end sequencing of the RPCI-23 mouse BAC library is being carried out by TIGR. For more online mouse resources see The Whole Mouse Catalog.
High Priority BAC sequencing
The identity of BAC clones determined to contain DNA sequence of particularly high biomedical interest can now be submitted to the NIH for priorty sequencing by the Mouse Genome Sequencing network. This effort is being coordinated by the Trans-NIH Mouse Initiative.
| Clone Nomenclature | ||||
|---|---|---|---|---|
| Library | Prefix | Plate | Row | Column |
| RPCI-23 | A | 0001-0480 | A-P | 01-24 |
| RPCI-24 | B | 0001-0288 | A-P | 01-24 |
Example: A0001P20
Marker Nomenclature
- All MIT STS' names remain the same e.g. D16Mit99
- Source name (see 4) and chromosome (if known) are attached in front
- For marker from GSC, St.Louis, numbers in front and "mus:" are trimmed off e.g. 32.MMHAP5FLC5 becomes MMHAP5FLC5, mus:AU22770 becomes AU22770
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Sources WU Washington University, Genome Sequencing Center, St.Louis RP Roswell Park Cancer Institue BCM Baylor College of Medicine AECOM Albert Einstein College of Medicine Genome Center BUC University of Pennsylvania (Dr. Maya Bucan and CBIL)
Example: 11-BCM-Aldh3p, WU-mm42f09.x1, X-RP-MTH2881
Sources of Marker Data
- Baylor College of Medicine (Dr. A. Bradley)
- Children's Hospital Oakland Research Institute (Dr. P. de Jong)
- Washington University, Genome Sequencing Center, St.Louis (Dr. J. McPherson)
- Albert Einstein College of Medicine Genome Center
- University of Pennsylvania (Dr. Maya Bucan and CBIL)
- Monell Chemical Senses Center
