Cryptococcus Neoformans
Summary
The Genome Sciences Centre, Vancouver, B.C., in collaboration with Dr. J. Kronstad, UBC, has fingerprinted 2 BAC libraries constructed from the H99 (serotype A) and JEC21 (serotype D) strains of Cryptococcus neoformans. The libraries were constructed by Research Genetics with DNA provided by the research group of Dr. Joe Heitman at Duke University. Sequences from the ends of BAC clones from both libraries have been generated. The fingerprint maps and the end sequences are being used in combination with shotgun sequence data for assembly of the genomic sequence at the Stanford Genome Technology Center. BAC fingerprinting and BAC end sequencing are supported by a grant from the Natural Sciences and Engineering Research Council of Canada (NSERC).
BAC Fingerprint Maps
Whole-clone HindIII fingerprints were generated using an improved version of the agarose gel-based methodology developed at the Washington University Genome Sequencing Center (Marra et al., 1997). The same technology has been used to produce a BAC fingerprint map of the mouse genome. Fingerprint data are stored in the form of an FPC database. The FPC software and the supporting documentation are available from the Sanger Centre.
Related links
- Stanford Genome Technology Center Whole genome shotgun of C.neoformans JEC21
- University of Oklahoma Advanced Center for Genome Technology (ACGT) JEC21 and H99 ESTs
- The Institute for Genomic Research (TIGR) Cryptococcus neoformans Database
BAC end sequences
A summary of the BAC end sequences, including the Phred quality scores, number of clones with 1 or both ends sequenced, and sequences from each library can be viewed here.