SSAKE

De novo Genome Assembler for Short DNA Sequence Reads

Project Description

SSAKE is a de novo assembler for short DNA sequence reads. It is designed to help leverage the information from short sequences reads by assembling them into contigs and scaffolds that can be used to characterize novel sequencing targets. SSAKE is the first published algorithm for genome assembly with short DNA sequences.

Genomic tools that use SSAKE algorithms

Algorithms of SSAKE are the core of many genomics applications (eg. VCAKE, QSRA, SHARCGS, SSPACE, JR-Assembler) and their design continues to inspire new-generation assemblers1 (eg. JR-Assembler, PNAS 2013). Applications of SSAKE extend beyond genome assembly and the technology was applied to profiling T-cell metagenomestargeted de novo assemblyHLA typing and was key to the discovery of Fusobacterium in colon cancer.

US National Marrow Donor Program / Be The Match

The US National Marrow Donor Program (NMDP)®/Be The Match® relies on SSAKE for HLA consensus assembly, which is cornerstone to their allele interpretation pipeline designed to match Donors and Recipients. The NMDP®/Be The Match® is a global leader in facilitating bone marrow and umbilical cord blood transplants to save the lives of patients with leukemia, lymphoma, genetic disorders, and other diseases.

 

Summary

SSAKE is written in PERL and runs on Linux. SSAKE cycles through short sequence reads stored in a hash table and progressively searches through a prefix tree for extension candidates. The algorithm assembled 25 to 300 bp [genome, transcriptome, amplicon] reads from viral, bacterial and fungal genomes.  SSAKE is lightweight, simple to setup & run and robust.

*Best performance is achieved by quality-trimming your reads before assembly (refer to the tools folder and SSAKE.readme/SSAKE.pdf and example assembly shell scripts in the test directory)

Enjoy SSAKE responsibly!

About the author: www.renewarren.ca

 

Experimental, NGS test data


1) Escherichia coli K12

A contiguous E.coli K12 assembly of quality-trimmed Illumina MiSeq PE300 in under 1 hour.

To download and assemble, run: ./test/MiSeqEcoliAssembly250XPE300.sh

E.coli K12 SSAKE assembly scaffold statistics with Illumina MiSeq
n (500)Average (bp)Largest (bp)N50 length (bp)Reconstruction (bp)
89 52,685 505,753 212,681 4,689,039

 

2) Campylobacter showae CC57C

Experimental, quality-trimmed, Illumina MiSeq sequence dataset (PE150, Colorectal cancer tumor isolate bacteria C.showae CC57C [PRJNA189774]) available for testing SSAKE:  ftp://ftp.bcgsc.ca/supplementary/SSAKE

To download and assemble, run: ./test/MiSeqCampylobacterAssembly.sh


If you use the data in your research, please cite:

Warren RL, Freeman DJ, Pleasance S, Watson P, Moore RA, Cochrane K, Allen-Vercoe E, Holt RA. 2013. Co-occurrence of anaerobic bacteria in colorectal carcinomasMicrobiome 1:16

 

3) Fusobacterium nucleatum CC53

We provide quality-trimmed HiSeq reads for a F.nucleatum CRC tumor isolate CC53.

To download and assemble, run: ./test/HiSeqFusobacteriumAssembly.sh

If you use the data in your research, please cite:

Castellarin M*, Warren RL*, Freeman JD, Dreolini L, Krzywinski M, Strauss J, Barnes R, Watson P, Allen-Vercoe E, Moore RA, Holt RA.  2012. Fusobacterium nucleatum infection is prevalent in human colorectal carcinoma. Genome Res. 22:299

 

Citing

If you use SSAKE in your research, please cite:

Warren RL, Sutton GG, Jones SJM, Holt RA. 2007 (epub 2006 Dec 8). Assembling millions of short DNA sequences using SSAKE. Bioinformatics 23:500


License

Copyright (c) 2006-2014 Canada's Michael Smith Genome Science Centre. All rights reserved.

This program is free software; you can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation; either version 2 of the License, or (at your option) any later version.

This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details.

Current Release
SSAKE 3.8.2

Released Apr 25, 2014

This release includes an option (-j) for adjusting the kmer length when running SSAKE in TASR mode (-s). A recent Illumina MiSeq dataset is available for testing SSAKE's performance: ftp://ftp.bcgsc.ca/supplementary/SSAKE
More about this release…

Download file Get SSAKE for all platforms
ssake_v3-8-2.tar.gz
If you are using Plone 3.2 or higher, you probably want to install this product with buildout. See our tutorial on installing add-on products with buildout for more information.

All Releases

Version Released Description Compatibility Licenses Status
3.8.2 Apr 25, 2014 This release includes an option (-j) for adjusting the kmer length when running SSAKE in TASR mode (-s). A recent Illumina MiSeq dataset is available for testing SSAKE's performance: ftp://ftp.bcgsc.ca/supplementary/SSAKE More about this release… GPL final
Project Resources

Project owner: Rene Warren