LaneRuler will identify lanes in a gel image. The core module is a command line c program, whose result can be reviewed and corrected using a java interface.
software package, named LaneRuler, has been developed for the
automated lane tracking in electrophoresis gels, with a focus on gels
for DNA fingerprinting. The lanes on such gels may not be straight
and parallel due to various reasons, and these deviations must be
accounted for in order to accurately size the restriction fragments
in each lane. In order to meet the high throughput requirements by
the projects at the British Columbia Cancer Agency Genome Sciences
Center (GSC), the software has capability to verify and correct its
results automatically, and prompting for user inspection only for
extremely abnormal cases. In validation testing using Bacterial
Artificial Chromosome (BAC) clones, the automatic lane tracking
results gave restriction fragment sizing results that are comparable
to those from manually supervised lane tracking results, achieving
sensitivity and specificity of restriction fragment identification
exceeding 95%. The current conception of the program is able to
successfully process 96% of the gels with no human intervention.
software has also given good results with electrophoresis gel for polymerase chain reaction (PCR) products and for cDNA.