ChopStitch

Exon annotation and splice graph reconstruction using transcriptome assembly and whole genome sequencing data

Project Description

ChopStitch is a new method for finding putative exons and constructing splice graphs using an assembled transcriptome and whole genome shotgun sequencing (WGSS) data. ChopStitch identifies exon-exon boundaries in de novo assembled RNA-seq data with the help of a Bloom filter that represents the k-mer spectrum of WGSS reads. The algorithm also detects base substitutions in transcript sequences corresponding to sequencing or assembly errors, haplotype variations, or putative RNA editing events. The primary output of our tool is a FASTA file containing putative exons. Further, exon edges are interrogated for alternative exon-exon boundaries to detect transcript isoforms, which are reported as splice graphs in dot output format.

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Current Release
ChopStitch 1.0.0

Released Feb 02, 2017

See ChopStitch GitHub page for details.
More about this release…

Download file Get ChopStitch for all platforms
ChopStitch-1.0.0

All Releases

Version Released Description Compatibility Licenses Status
1.0.0 Feb 02, 2017 See ChopStitch GitHub page for details. More about this release… GPLv3 for non-commercial usage
GPLv3 for non-commercial usage
GPLv3 for non-commercial usage
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