This is a short tutorial on the LaneRuler software suite. The core module is a command line c program, whose result can be reviewed and corrected using a java interface. The tutorial will go through the flow of the program, various flags for specifying the format of the gel, and details about all the options available.
Sample lane tracking result for a cDNA gel.
Sample lane tracking result for a PCR gel.
Sample lane tracking result for a DNA fingerprinting gel.
Ran with default parameters. Function call: >findlanes -p <project_directory>
Due to low data density, toneadjustment was turned off. Function call: >findlanes -p <project_directory> -a
A rotation was required as the gel initially had wells at top edge of the image. Due to low number of marker lanes, we match all peaks in all zones (-i). Tone adjustment was not done due to high data signal (-a). The morphology element size was adjusted since the marker band separation to image width ratio has changed significantly. Function call: >findlanes --numlanes 11 --markerperiod 10 --orientation 1 -a -i --morphelement 150