Rebuilding microbial genomes
Rebuilding microbial genomes.
DNA can be isolated or directly synthesized and molded into a synthetic genome using existing genomic blueprints and molecular biology tools. Activating such a synthetic genome will yield a synthetic cell. In this publication we examine obstacles associated with this latter approach using a model system whereby a donor genome from H. influenzae is fragmented, and the pieces are then modified and reassembled stepwise in an E. coli host cell.
Holt Ra, Warren R, Flibotte S, Missirlis PI & Smailus DE. Bioessays 2007. 29(6):580-590.
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