Profiling model T cell metagenomes
T cell receptor (TCR) diversity in peripheral blood has not yet been fully profiled with sequence level resolution. Each T cell clonotype expresses a unique receptor, generated by somatic recombination of TCR genes and the enormous potential for T cell diversity makes repertoire analysis challenging. We developed and present a sequencing approach and assembly software (immuno-SSAKE or iSSAKE) for profiling T cell metagenomes using short reads from the massively parallel sequencing platforms.
Warren RL, Nelson BH, Holt RA. 2009 Profiling model T cell metagenomes with short reads. Bioinformatics. 25(4):458-64. [Epub 2009 Jan 9] PMID: 19136549 doi:10.1093/bioinformatics/btp010