New sequencing technologies such as Solexa/Illumina sequencing provide an ideal method for measuring the abundance of small RNA molecules in specialized cell types.  In this article in Genome Research, Ryan Morin describes novel techniques for analysis of Solexa/Illumina small RNA libraries and reveals the differences in the microRNA repertoire of human embryonic stem cells during differentiation.  This article also describes many previously undiscovered human microRNA genes, leading to the suggestion that many novel microRNAs will be discovered in the near future.


Full citation

Morin RD, O'Connor MD, Griffith M, Kuchenbauer R, Delaney A, Prabhu AL, Zhao YJ, McDonald H, Zeng T, Hirst M, Eaves CJ and Marra MA.  2008.  Application of massively parallel sequencing to microRNA profiling and discovery in human embryonic stem cells.  Genome Res, 18(4):610-21.

Further details of this publication can be found online at:

http://genome.cshlp.org/cgi/content/abstract/18/4/610

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